Alleviation of γ-enolase decrease by the chlorogenic acid administration in the stroke animal model

Stroke is a major cause of death and long-term disability. Chlorogenic acid phenolic compound with potent neuroprotective effect. γ-enolase phosphopyruvate hydratase found in mature neurons plays an important role the neuronal survival. This study investigated whether chlorogenic regulates expression during cerebral ischemia. Middle artery occlusion (MCAO) was performed to indcue Adult male rats were used (30 mg/kg) or phosphate buffered saline (PBS) injected intraperitoneally 2 hours after MCAO surgery. Cerebral cortical tissues collected 24 Our proteomic approach identified reduction caused by damage mitigation this treatment. Results reverse transcription-polymerase chain reaction Western blot analyses showed decrease PBS-treated group. However, treatment attenuated decrease. immunofluorescence staining change These results demonstrated that MCAO-induced Therefore, we suggest mediates function regulating ischemia may be as therapeutic agent for brain diseases including stroke.